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Thromb Haemost 1968; 19(01/02): 161-168
DOI: 10.1055/s-0038-1651192
DOI: 10.1055/s-0038-1651192
Originalarbeiten – Original Articles – Travaux Originaux
A Method to Correct for the Continuing Activation during the Second Stage in a Two-Stage Assay
Exemplified by Urokinase Activation of Plasminogen Determined by the Lysis Time MethodFurther Information
Publication History
Publication Date:
27 June 2018 (online)
Summary
In coagulation and fibrinolysis, kinetic data are difficult to obtain with ordinary quantitative methods because no simple means are available to stop the reaction before the determination of the activity formed.
This work describes how to calculate, from the data recorded, the amount of activity at the moment the determination is started.
A formula is given for a zero, a first and a second-order reaction.
The method is exemplified by urokinase activation of plasminogen into plasmin. The determination of the plasmin activity is done by means of the lysis time method.
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References
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